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KMID : 0613820190290010084
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Journal of Life Science
2019 Volume.29 No. 1 p.84 ~ p.89
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Antimicrobial Activity of Pseudomonas aeruginosa BCNU 1204 and Its Active Compound
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Shin Hwa-Jin
Joo Woo-Hong
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Abstract
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Previous screening of novel antibacterial agents revealed that some bacterial isolates exhibited antibiotic activity against both gram-positive and gram-negative bacteria and that they showed antibacterial activity, even against methicillin-resistant Staphylococcus aureus (MRSA). Among these isolates, one bacterial strain, BCNU 1204, was identified as Pseudomonas aeruginosa using phenetic and phylogenetic analysis, based on 16S ribosomal RNA gene sequences. The maximum productivities of antimicrobial substances of BCNU 1204 were obtained after being cultured at 35°C and pH 7.0 for 4 d in King’s medium B (KMB). Dichloromethane (DCM) and ethylacetate (EA) extracts of P. aeruginosa BCNU 1204 exhibited strong antimicrobial activity, particularly against gram-positive bacteria. The EA extracts exhibited broad-spectrum activity against antibiotic resistant strains. Fraction 5-2, was obtained by recycling preparative liquid chromatography (LC) and preparative thin-layer chromatography (TLC) and was identified as phenazine-1-carboxylic acid belonging to phenazines using gas chromatography and mass spectrometry (GC/MS). Its minimum inhibitory concentration (MIC) values were 25 μg/ml, 50 μg/ml, ≥25 μg/ml, and ≥50 μg/ml for MRSA CCARM 3089, 3090, 3091, and 3095 strains, respectively. P. aeruginosa BCNU 1204 may be a potential resource for the development of anti-MRSA antibiotics. Additional research is required to identify the active substance from P. aeruginosa BCNU 1204.
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KEYWORD
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Antimicrobial activity, methicillin-resistant Staphylococcus aureus (MRSA), phenazine compounds, phenazine-1-carboxylic acid, Pseudomonas aeruginosa
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